Fig. 4: Relationship between DKP stereoisomerase NozR and representative Asp/Glu racemase family members33,34,35,36,37,38. | Nature Communications

Fig. 4: Relationship between DKP stereoisomerase NozR and representative Asp/Glu racemase family members33,34,35,36,37,38.

From: Unveiling an indole alkaloid diketopiperazine biosynthetic pathway that features a unique stereoisomerase and multifunctional methyltransferase

Fig. 4

a An unrooted neighbor-joining tree supports the phylogenetic relationship of NozR with representative functionally characterized members of the Asp/Glu racemase family. Species names and protein accession numbers for these isomerases are color-coded to match schematics of typical reactions catalyzed by each. b In prototypical reactions catalyzed by Asp/Glu racemases, one residue of the catalytic dyad (e.g. Cysa) deprotonates the α-H of a stereoisomer to yield an enolate intermediate, which is reprotonated via the other catalytic dyad residue (e.g. CysB) from the opposite face of the molecule to yield reversible inversion of configuration.

Back to article page