Fig. 7: In vitro functional assays revealed that NozMT catalyzes N1’- followed by C3-methylation as the final steps in nocardioazine B assembly. | Nature Communications

Fig. 7: In vitro functional assays revealed that NozMT catalyzes N1’- followed by C3-methylation as the final steps in nocardioazine B assembly.

From: Unveiling an indole alkaloid diketopiperazine biosynthetic pathway that features a unique stereoisomerase and multifunctional methyltransferase

Fig. 7

a Incubation of purified recombinant NozMT with 6 or 7 and SAM yielded nocardioazine B (2), with [M + H]+ m/z 469 by ESI+. LC was conducted with a C18 stationary phase and gradient mobile phase of H2O/MeCN (40–90%) containing 0.1% HOAc, as detailed in Methods. b The time course of reactant 6, N1’-methylated intermediate 7, and product 2 relative concentrations during the NozMT-catalyzed reaction supports successive N1’- and C3-methylation (data points indicate means for n = 2 independent replicates). c Postulated mechanism of sequential methylation reactions of 6 catalyzed by NozMT. Source data are provided as a Source Data file.

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