Fig. 4: QER7 suppresses transcription of CCM genes and depends on PHOT.

qer7, phot, and cia5 cells alongside with their complemented lines (qer7-C, phot-C, and cia5-C respectively) and WT backgrounds, were synchronized under 12 h light (15 µmol m⁻² s⁻¹)/12 h dark cycles, in photoautotrophic conditions (Sueoka’s high salt medium; HSM), at 23 °C in Erlenmeyer flasks shaken at 125 rpm. After sampling at the end of the dark phase, cells were exposed to 300 µmol photons m⁻² s⁻¹ (HL) and samples were taken 1 h after HL exposure. a Relative expression levels of CCM genes at the indicated conditions. b Relative expression levels of QER7 and LCR1 in WT, phot and phot-C. c Relative expression levels of QER7 in WT, cia5 and cia5-C. In all cases (a–c) expression levels were normalized to WT LL (n = 3 biological samples, mean ± sd). The two-sided p values for the comparisons are based on ANOVA employing Dunnett’s multiple comparisons test on log10-transformed values and are indicated in the graphs (*P < 0.005, **P < 0.01, ***P < 0.001, ****P < 0.0001). The exact p values are shown in the Source Data file.