Fig. 1: Analysis of chromatin-associated factors for improving GBE and ABE editing.
From: HMGN1 enhances CRISPR-directed dual-function A-to-G and C-to-G base editing
a Schematic of GBE, ABE, and GGBE binding to DNA. b Base editing efficiency of C-to-G across GBE and GBE variants fused with 16 chromatin-associated factors at HIRA and EMX1-site1 loci in HEK293T cells. c Base editing efficiency of A-to-G across A8e and A8e variants fused with 16 chromatin-associated factors at HIRA and EMX1-site1 loci in HEK293T cells. d Base editing efficiency of C-to-G between GBE and HMGN1-GBE at eight genomic loci in HEK293T cells. e Base editing efficiency of A-to-G between A8e and HMGN1-A8e at eight genomic loci in HEK293T cells. CMF chromatin-associated factors, Ac acetylation, Me methylation, Ub ubiquitination. Mean ± SEM (b–e) of all individual values of sets of n = 3 independent replicates are shown. All statistical analysis for samples were conducted using unpaired Student’s t test (two-tailed) in GraphPad Prism 8. Source data are provided as a Source Data file.
