Fig. 3: Phylogenetic diversity of flaviviruses identified here.

a Phylogeny of the Flaviviridae based on amino acid sequences of the NS5 gene. Viruses identified in this study are color-coded according to the animal from which they were sampled. The tree was rooted with Tamana bat virus. Scale bar depicts the number of amino acid substitutions per site. b Phylogeny of pestiviruses based on the amino acid sequences of the NS5 gene. Representatives of Pestivirus A-K are incorporated. The tree was rooted with Pestivirus K and BaPeV. Viruses detected in the different tissues of the pangolins are labeled in different colors. c Viral particles of pangolin pestivirus, grown in Vero E6 cell culture, seen by transmission electron microscopy image. Scale bar, 2 μm. The experiment was performed independently in triplicate with similar results.