Fig. 4: PWAS pesticides are directly toxic to PD patient-derived mDA neurons in a live imaging screen. | Nature Communications

Fig. 4: PWAS pesticides are directly toxic to PD patient-derived mDA neurons in a live imaging screen.

From: A pesticide and iPSC dopaminergic neuron screen identifies and classifies Parkinson-relevant pesticides

Fig. 4

a Scatter plot with the number of THtdTomato+ cells measured by live imaging analysis 11 days after the first treatment. DMSO controls (green data points) were present on each assay plate. Water control (green data points) was present on the assay plate containing primarily water-soluble pesticides. Rotenone (red data points) and ziram (red data points) were used as positive controls. Blue data points represent the different pesticides from the PWAS study. Horizontal line denotes three standard deviations below DMSO mean. b Upper image is a x10 magnification live image of a DMSO control well. Lower image is from a propargite treated well. Scale bar =  100 µM. More than five independent experiments repeated with DMSO and propargite treatment showed similar neuronal morphology with DMSO and similar extent of cell death and debris with propargite. c Four concentration dose curves of PWAS toxicants producing death in SNCA triplication THtdTomato sorted neurons. Cell numbers measured by high-content imaging of live cultures 11 days after first treatment. n = 1 with two technical replicates per water soluble pesticide and three technical replicates per DMSO and ethanol soluble pesticides per dose per pesticide for the screen dose curves. Red lines connect average cell number value for each pesticide concentration. Source data are provided as a Source Data file.

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