Fig. 7: Translation activities of Nluc mRNAs in cultured mammalian cells. | Nature Communications

Fig. 7: Translation activities of Nluc mRNAs in cultured mammalian cells.

From: Cap analogs with a hydrophobic photocleavable tag enable facile purification of fully capped mRNA with various cap structures

Fig. 7: Translation activities of Nluc mRNAs in cultured mammalian cells.The alt text for this image may have been generated using AI.

Capped mRNAs were introduced into HeLa cells (a‒c, e) or JAWS II cells (d, f) using Lipofectamine MessengerMAX reagent. Cells were cultured for the indicated time, lysed, and the Nluc expression was measured. The cap analog used for mRNA preparation is indicated in the graph as the name of the mRNA. AnP means the mRNA was further treated with Antarctic phosphatase to remove its 5’ phosphate. Data were mean ± s. e. m. for biological replicates (n = 4). a, b The statistically significant differences for the capped RNA samples in one-way ANOVA followed by Tukey’s test were marked as follows. ns, >0.05; *p < 0.05; **p < 0.01; ***p < 0.001. c‒f The statistically significant differences for the capped RNA samples from “ARCA” (c, d) or “Tri_1” (e, f) in one-way ANOVA followed by Dunnett’s test were marked as follows. ns, >0.05; *p < 0.05; **p < 0.01; ***p < 0.001. a‒f Each experiment was repeated independently twice (a, b) or at least three times (c‒f) to obtain similar results. Source Data are provided with this paper.

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