Table 1 Summary of translation activity of Nluc mRNAs evaluated in this study

Name of mRNA^a	Capping efficiency (%)^b	Normalized Nluc expression^c
Name of mRNA^a	Capping efficiency (%)^b	HeLa cells	JAWS II cells	Mice liver	Mice spleen
Dinucleotide Cap analogs (m⁷G-ppp-G)
ARCA	56	1.00 ± 0.02	1.00 ± 0.03	n.d.	n.d.
ARCA/ AnP	56	0.97 ± 0.03	1.13 ± 0.03	n.d.	n.d.
DiPure	>99	2.36 ± 0.05	3.51 ± 0.09	n.d.	n.d.
DiPure/3′OMe	>99	2.27 ± 0.07	3.11 ± 0.21	n.d.	n.d.
DiPure/2′OMe	>99	2.01 ± 0.09	2.58 ± 0.08	n.d.	n.d.
Tri/ tetranucleotide cap analogs m⁷G-ppp-AG(G)
Tri_1	87	1.00 ± 0.04	1.00 ± 0.05	1.00 ± 0.15	1.00 ± 0.13
Tri_1/ AnP	87	1.07 ± 0.03	1.51 ± 0.06	1.47 ± 0.23	0.61 ± 0.08
Tetra_2	52	0.52 ± 0.02	0.70 ± 0.04	n.d.	n.d.
Tetra_2/AnP	52	0.51 ± 0.02	0.53 ± 0.02	n.d.	n.d.
TriPure_0	>99	1.12 ± 0.06	1.04 ± 0.08	n.d.	n.d.
TriPure_1	>99	1.31 ± 0.05	1.07 ± 0.05	0.80 ± 0.08	0.90 ± 0.07
TetraPure_2	98	1.91 ± 0.06	2.99 ± 0.19	4.86 ± 0.61	2.48 ± 0.29
TetraPure_2/m6A	95	1.98 ± 0.07	3.60 ± 0.19	4.07 ± 0.48	3.58 ± 0.63

^aThe.RNAs were named after the cap analog used. AnP means the RNA was further dephosphorylated using Antarctic phosphatase.
^bThese values are taken from Figs. 2f, 4g.
^cThese data are calculated from the data shown in Figs. 6c–f, 7, represented as means ± s. e. m. The activities were normalized to the control samples that were ARCA for mRNAs prepared with a dinucleotide cap analog or Tri_1 for mRNAs prepared with a tri/tetranucleotide cap analog. All data were considered for evaluation If the activity was measured at more than one time point.

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