Fig. 1: Comparison of workflows for Cryo EPt-SPA (Left column) and TEM-SPA (Right column). | Nature Communications

Fig. 1: Comparison of workflows for Cryo EPt-SPA (Left column) and TEM-SPA (Right column).

From: Cryogenic electron ptychographic single particle analysis with wide bandwidth information transfer

Fig. 1

Schematic diagrams of the optical configuration a and data acquisition for ptychography b. c Typical diffraction patterns and corresponding probe positions used for ptychography (blue). Ptychographic reconstructed amplitude d and phase e of an object function. Instances of amplitude f and phase g of particles picked from d and e, respectively. h Schematic diagram of the optical configuration for TEM data acquisition. i Movie frames collected in TEM mode (left) and translations calculated for individual frames (right). j Motion-corrected image from a movie. k CTF correction by fitting the amplitude spectrum of j. l Picked particles instances from j.

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