Fig. 4: Substrate recognition by SIN3B.
From: Mechanism of assembly, activation and lysine selection by the SIN3B histone deacetylase complex
a Overview of the SIN3BFL complex cryo-EM density at 3.4 Å resolution. PHF12 (magenta) is highlighted here and its PHD finger domains are shown in close up view in (b, PHD1) or (d, PHD2). b Close-up view on the PHF12 interactions with MORF4L1 MRG domain. PHF12PHD1 recognises a composite interface of MRG and the PHF12 SIN3B interaction domain 1 (SID1). Cryo-EM map of the SIN3BFL reconstruction is here shown lowpass filtered to 7 Å for better showing the PHF12PHD1 domain (left) and the overall lowpass filtered map is shown on the right. c The cryo-EM map from (a) is shown rotated 90 degrees to highlight the interactions of PHF12SID2 with SIN3BPAH3 and MORF4L1 MRG domain. d Close-up view on the PHF12PHD2 interacting with SIN3B and HDAC2. Key residues involved in these interactions, zinc (Zn) atoms and their cryo-EM densities are depicted.
