Fig. 8: Adhesion, AMPK and mitochondrial dynamics in vivo and in primary melanoma tissues. | Nature Communications

Fig. 8: Adhesion, AMPK and mitochondrial dynamics in vivo and in primary melanoma tissues.

From: AMPK is a mechano-metabolic sensor linking cell adhesion and mitochondrial dynamics to Myosin-dependent cell migration

Fig. 8: Adhesion, AMPK and mitochondrial dynamics in vivo and in primary melanoma tissues.The alternative text for this image may have been generated using AI.

a, b Representative immunohistochemistry (IHC) images and quantification of DDR1, MFN2, OPA1, pAMPK and pMLC2 in subcutaneous tumours in mice from WM983A, WM983B, A375P or A375M2 cells (n = 8 mice/group). Scale bar = 100 μm. c, d Representative QuPath mark-up images and quantification of DDR1, MFN2, OPA1 and pAMPK expression and amoeboid score (calculated as indicated in methods) in matched tumour body (TB) and invasive front (IF) of primary tumours from human melanoma tissue microarrays (n = 46 tumours). Scale bar = 200 μm. e Representative IHC images showing DDR1, MFN2, OPA1 and pAMPK staining in elongated cells from TB and rounded cells from IF. Scale bar = 50 μm. Box plots (b, d) show median (centre line), interquartile range (box) and min-max values (whiskers). p values were calculated using two-tailed tests (b, d). p value by unpaired t-test (b) and Wilcoxon test (d). Source data are provided as a Source Data file. See also Supplementary Fig. 9.

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