Fig. 1: VGLUTPM anion currents are pH- and voltage-dependent.
From: Vesicular glutamate transporters are H+-anion exchangers that operate at variable stoichiometry
a Representative recordings from a transfected cell with Cl− as the internal anion perfused with Cl−-based solutions. No leakage subtraction was performed, and red lines represent zero current. b Plot of uncorrected whole-cell current amplitudes at the end of 500 ms voltage steps to −160 mV versus whole-cell fluorescences. Lines and shaded areas show the mean and 95% CI from linear fits. Measurements at pH 5.5 are given by large symbols, measurements at 7.4 by small symbols. Insets on the left show representative current responses to voltage steps between −160 and +80 mV at external pH 5.5 and 40 mM external Cl− with indicated internal anions. c Voltage dependence of background corrected whole-cell currents normalized to whole-cell fluorescence (means ± CI, n = 10 cells for each internal anion). d External pH dependence of maximum current amplitudes at −160 mV (means ± CI, n = 27 cells with internal Cl−, n = 12 cells with internal NO3−). Lines and shaded area depict mean and 95% CI from fits with Michaelis-Menten relationships. Source data are provided in the Source Data file.
