Fig. 2: VGLUT1PM functions as voltage-gated anion channels.
From: Vesicular glutamate transporters are H+-anion exchangers that operate at variable stoichiometry
a Averaged power spectrum from 13 cells expressing VGLUT1PM with internal Cl− at pH 5.5 after subtraction of background currents. The red line represents the fit with a second-order Lorentzian function in addition to pink noise (S1 = 9.2 × 10–28 A2/Hz; S2 = 6.2 × 10–28 A2/Hz, a = 1.36), and black lines the individual components of the fit. b Representative current–variance plots of stationary noise analysis at −160 mV with internal Cl− (red) or NO3- (black). c, d Unitary current amplitudes (c) and absolute open probabilities (d) at −160 mV for cells dialyzed with internal Cl− (red, n = 15 cells) or NO3− (black, n = 13 cells). P-values from two-tailed t-test. Box plots show single data as symbols, mean values as dashed lines, medians as solid lines, upper and lower quartiles as box borders and 95% CI as whiskers. e Changes in standard deviation (STD) of experimental current recordings upon filtering with Butterworth filters of different low-pass filter frequencies (symbols, n = 6 patches) and of simulated unitary current recordings with indicated open times (lines). Currents were obtained from excised outside-out patches with NO3− as anion in the pipette solution at −160 mV; the inset depicts a representative recording. f Representative responses of whole-cell currents with internal Cl− to voltage steps consisting of an activating prepulse to −160 mV followed by variable test pulses. Source data are provided in the Source Data file.
