Fig. 2: Two subpopulations of basophils are also detected in the bone marrow while only one of them in the peripheral blood and spleen.

a CD200R3⁺CD49b⁺ basophils among the cKit^- population in the peripheral blood, spleen, and bone marrow were gated (left panels), and surface expression of FcεRIα and CD49b (middle panels) and that of CLEC12A and CD9 (right panels) are shown. b CLEC12A^loCD9^hi and CLEC12A^hiCD9^lo subpopulations of basophils in the bone marrow and CLEC12A^loCD9^hi basophils in the spleen and peripheral blood were separately sort-purified and stained with May-Grünwald Giemsa (scale bar, 10 μm). c Scatter plots of CLEC12A^hiCD9^lo (blue dots) and CLEC12A^loCD9^hi (red dots) subpopulations in the bone marrow are shown. d The surface expression of CD34 in each subpopulation is shown. Open histograms indicate control staining with isotype-matched control. e–i Basophils were isolated from the bone marrow and spleen of Mcpt8^GFP mice (Supplementary Fig. 5) and separately subjected to scRNA-seq analysis. In (e), UMAP plots of combined data set of 2745 cells from bone marrow basophils and 731 cells from spleen basophils are shown. In (f), violin plots of the expression of indicated genes in each cluster are shown. In (g), RNA velocity analysis was conducted on the combined dataset. UMAP plot colored by scVelo latent time is shown. In (h), Monocle3 pseudotime analysis was conducted on the combined data set. UMAP plot colored by pseudotime is shown. In (i), gene expression changes of indicated genes along with the pseudotime ordering (indicated in Fig. 2h) are shown. Data shown in (a–d) are representative of at least three independent experiments. Data in (e–i) were obtained from a single experiment.