Fig. 6: Model of EGFR compartment transitions.
From: Confinement of unliganded EGFR by tetraspanin nanodomains gates EGFR ligand binding and signaling
A EGFR (blue) compartmentalization and ligand-binding model. Unliganded EGFR outside of nanodomains cannot become ligand-bound. EGFR can transition into a tetraspanin domain (turquoise), where EGF (orange) binding is permitted. Unliganded EGFR slowly exit a tetraspanin domain, while ligand-bound EGFR rapidly depart a tetraspanin domain and can be confined to a clathrin domain and removed from the membrane. The ki describe the rates of transition between compartments. B shows the confined EGFR fractions from a stochastic quantitative model of EGFR diffusion and ligand binding dynamics on the membrane, following (A). The black solid line represents the fraction of all EGFR that are confined to nanodomains, comparable to immobile+confined fraction in the left panel of Fig. 1B. The blue solid line represents the fraction of liganded EGFR confined to nanodomains, comparable to the immobile+confined fraction in the right panel of Fig. 1B. Red and magenta dashed lines represent the fraction of all EGFR confined to tetraspanin and clathrin nanodomains, respectively, comparable to the control data in Fig. 2C. This data is for slow unliganded EGFR entry and exit from tetraspanin nanodomains. Each data point is averaged over 100 EGFR trajectories. Statistical analysis and p-values are indicated in Supplementary Table 1. Source data are provided as a Source Data file.
