Fig. 1: snRandom-seq for FFPE tissues overview. | Nature Communications

Fig. 1: snRandom-seq for FFPE tissues overview.

From: High-throughput single nucleus total RNA sequencing of formalin-fixed paraffin-embedded tissues by snRandom-seq

Fig. 1

The workflow of snRandom-seq for FFPE tissues includes FFPE sample selection, paraffin dissolution, single nuclei isolation, and permeabilization, single-strand DNAs blocking, reverse transcription, dA tailing, droplet barcoding, primers releasing and extension, droplets breaking and PCR amplification, and sequencing. Red dashed circle in the FFPE tissue block: the areas of interest. Blue dashed box: the three in situ reactions, including single-strand DNAs blocking, reverse transcription, dA tailing. AAA: dA tail in the 3′ of cDNA. TTT: poly(dT) in the poly(dT) barcoded primers. Gray arrows: the direction of extension.

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