Fig. 3: Verification plots of nonmalignant epithelium and malignant epithelium after identification.
a Box plot showing distribution of cancer-related scores (average expression levels of cancer-related epithelial marker genes) for cells categorized as nonmalignant epithelial cells and M/PDA cells (t-test, p < 2.2 × 10−16). b Box plot showing distributions of cancer-related scores (average expression levels of cancer-related epithelial marker genes) for cells categorized as SRCC and mucous cells (t-test, p < 2.2 × 10−16). c Heatmap showing inferCNV for all subclusters of epithelial cells. Red: amplifications; blue: deletions. d Histogram showing CNV score plot of all subclusters of epithelial cells. e Box plot showing CNV score plot of epithelial cells by cell type. The CNV score of SRCC cells was lower than that of M/PDA and mucous cells, while higher than that of nonmalignant epithelial cells (t-test, p < 2.2 × 10−16). f GSVA of SRCC and mucous cells. SRCC cells were mainly enriched in cancer-related signalling pathways such as the TNF-α signalling pathway, NF-κB signalling pathway, and TGF-β signalling pathway. GSVA data were plotted according to the t value of limma, and at value > 5 was considered significant. The statistical strategy were two-sided Student’s t-test. In a, b, d and e, All specimens were participated in the analysis (n = 18), Data are presented as mean values ± SEM. In the box plot, the black dots represent outliers, the error bars represent SEM, the box midpoints represent means and the boxes represent inter-quartile positions. p values were calculated using the two-sided unpaired Student’s t-test, P values < 0.05 were considered to indicate significance: **p < 0.01; ***p < 0.001. Source data are provided as a Source Data file.
