Fig. 4: BA-specific deletion of c-Kit inhibits the denervation-induced lipid accumulation and reduces SCD1 in the BAs of BAT. | Nature Communications

Fig. 4: BA-specific deletion of c-Kit inhibits the denervation-induced lipid accumulation and reduces SCD1 in the BAs of BAT.

From: Endothelial cell-derived stem cell factor promotes lipid accumulation through c-Kit-mediated increase of lipogenic enzymes in brown adipocytes

Fig. 4

a Diagram of the experimental procedure for the unilateral denervation of BAT in 8-week-old control (Con) and c-Kit∆BA mice, and harvest BAT (I-BAT and D-BAT) 3 or 5 days later for the analyses. b, c Representative immunoblotting and comparisons of c-Kit, ACL, ACC, FASN, and SCD1 in the I-BAT and D-BAT 3 days after the unilateral denervation (I-BAT3 or D-BAT3). The same amount of protein loading in each lane is verified by immunoblotting of tubulin. Each dot indicates a value from one mouse and n = 6 mice/group from two independent experiments. Horizontal and vertical bars indicate mean ± SD. *,#P < 0.05 versus Con I-BAT3 or c-Kit∆BA I-BAT3 by one-way ANOVA test followed by Tukey’s post-hoc test. Protein sizes are indicated as kilodalton (kDa). d, e Representative images and comparisons of the populations of different sizes of lipid droplets in the CD147+ BAs of BAT among the indicated groups. White arrowheads indicate the lipid droplets whose size is larger than 51 µm2 in CD147+ BA. Scale bars, 20 µm. Each dot indicates the population (%) of the total of 1200–2400 droplets (100%) from three portions of BAT in one mouse and n = 5 mice/group from two independent experiments. Vertical bars indicate mean ± SD. *P < 0.05 by two-way ANOVA test followed by Sidak test. NS not significant.

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