Fig. 4: L-citrulline formation by recombinant DDAH. | Nature Communications

Fig. 4: L-citrulline formation by recombinant DDAH.

From: A multicentric consortium study demonstrates that dimethylarginine dimethylaminohydrolase 2 is not a dimethylarginine dimethylaminohydrolase

Fig. 4

Recombinant DDAH and GST proteins were incubated with 10 mM ADMA, and the enzymatic activity was measured by detection of L-citrulline production. The DDAH specific enzymatic activity of rDDAH1 was 17.10 ± 0.4 (pmoles/min/µg ± SD). The experiments with recombinant DDAH2 30 µg and GST 30 µg were performed twice while the remaining experiments with the other conditions were performed three times. Kruskal–Wallis test with Dunn’s multiple comparison test to the blank was performed. n = 9 for Blank, 3 µg GST protein, 3 µg rDDAH1, 3 µg rDDAH2; n = 6 for 30 µg GST protein, 30 µg rDDAH2 (technical replicates). Data are presented as mean ± SD. **p < 0.01 (p = 0.0035). Source data are provided as a Source Data file.

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