Fig. 5: ADMA metabolism by HEK293T cells overexpressing DDAH1 and DDAH2. | Nature Communications

Fig. 5: ADMA metabolism by HEK293T cells overexpressing DDAH1 and DDAH2.

From: A multicentric consortium study demonstrates that dimethylarginine dimethylaminohydrolase 2 is not a dimethylarginine dimethylaminohydrolase

Fig. 5

a–c mRNA and protein expression of HEK293T cells stably transfected with DDAH1 or DDAH2 in comparison to wild-type cells. d HEK293T cells with DDAH1 or DDAH2 overexpression were used to measure citrulline formation, a product of ADMA metabolism, at increasing substrate concentrations. Kruskal–Wallis test with Dunn’s multiple comparison test to the wild-type cell line was performed for DDAH1 mRNA expression analysis while one-way ANOVA with multiple comparisons to the wild-type cell line was performed for DDAH2 mRNA expression analysis (n = 3 biological replicates). DDAH activity assay using ADMA performed with n = 3 and n = 2 (biological replicates) respectively for DDAH1 and DDAH2 HEK293T cell models. Data are presented as mean ± SE. a *p < 0.001 (p = 0.0146); b ****p < 0.0001 (p < 0.000001). Source data are provided as a Source Data file.

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