Fig. 8: DDAH activity assay of tissues from wild type and Ddah1 knockout mice. | Nature Communications

Fig. 8: DDAH activity assay of tissues from wild type and Ddah1 knockout mice.

From: A multicentric consortium study demonstrates that dimethylarginine dimethylaminohydrolase 2 is not a dimethylarginine dimethylaminohydrolase

Fig. 8

a, b mRNA expression of DDAH isoforms in tissues of wild type and Ddah1 knockout mice. c Representative Western blot image of protein expression in tissue lysates from wild type and Ddah1 knockout mice. d Level of D7-labelled citrulline produced from the metabolism of D7-labelled ADMA by tissues of wild type and Ddah1 knockout mice. Statistical analyses were performed by unpaired two-sided Mann–Whitney test for non-normally distributed data with comparisons to the wild-type group for each tissue type (n = 5 biological replicates). Outliers were removed by ROUT method (Ddah1 KO liver, Ddah1 KO kidney, n = 1 from Ddah1 mRNA expression analysis). Data are presented as mean ± SE. a *p < 0.05 (p = 0.0159), **p < 0.01 (p = 0.0079), ***p < 0.001 (heart p = 0.0003, lung p = 0.0006); d **p < 0.01 (p = 0.0079). Source data are provided as a Source Data file.

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