Fig. 9: DDAH activity assay of tissues from wild type and Ddah2 knockout mice. | Nature Communications

Fig. 9: DDAH activity assay of tissues from wild type and Ddah2 knockout mice.

From: A multicentric consortium study demonstrates that dimethylarginine dimethylaminohydrolase 2 is not a dimethylarginine dimethylaminohydrolase

Fig. 9

a, b mRNA expression of DDAH isoforms in tissues of wild type and Ddah2 knockout mice. c Representative Western blot image of protein expression in tissue lysates from wild type and Ddah2 knockout mice. d Level of D7-labelled citrulline produced from the metabolism of D7-labelled ADMA by tissues of wild type and Ddah2 knockout mice. Statistical analysis was performed using two-sided unpaired t-test for normally distributed data (a, b) and unpaired two-sided Mann–Whitney test for non-normally distributed data (d), with comparisons to the wild-type group of each tissue type (n = 5 biologically replicates). Data are presented as mean ± SE. ***p < 0.001 (p = 0.0001), ****p < 0.0001. Source data are provided as a Source Data file.

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