Fig. 2: Evolved sdrM alleles increase DLX resistance and evolvability. | Nature Communications

Fig. 2: Evolved sdrM alleles increase DLX resistance and evolvability.

From: Efflux pump gene amplifications bypass necessity of multiple target mutations for resistance against dual-targeting antibiotic

Fig. 2

a DLX MICs of WT, and the three sdrM allelic replacement mutants in M63. Data shown are the mean ± standard deviation of five biological replicates. Significance is shown for comparison to the WT, or between mutants as indicated, as tested by a one-way ANOVA with Tukey’s test for multiple comparisons (*P < 0.05, P for WT vs sdrM1 = 0.0138, WT vs sdrM2 = 0.0486, **P < 0.01, sdrM1* vs sdrM3* P = 0.0015, ***P < 0.001, sdrM2* vs sdrM3* P = 0.0004, ****P < 0.0001). b Normalized fluorescence (intrinsic fluorescence of DLX/OD600) was measured for the indicated strains, as a proxy for the intracellular DLX concentration. Data shown are the mean ± standard error of three biological replicates. c Percentage of the 12 independent populations of the indicated strains that evolved DLX resistance over time when evolved in 2.5x the respective DLX MICs. Source data are provided in the Source Data file.

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