Fig. 5: The presence of sdrM facilitates the evolution of DLX resistance. | Nature Communications

Fig. 5: The presence of sdrM facilitates the evolution of DLX resistance.

From: Efflux pump gene amplifications bypass necessity of multiple target mutations for resistance against dual-targeting antibiotic

Fig. 5

a DLX MICs of the WT and sdrM::Tn strains in M63. Data shown are the mean ± standard deviation of three biological replicates. Significance is shown for comparison to the WT as tested by an unpaired two-tailed t test (*P = 0.0112). b The presence of mutations in genes encoding DNA gyrase subunits (gyrA, gyrB) and DNA topoisomerase IV subunits (parC, parE), the three mutant alleles sdrM1*, sdrM2*, and sdrM3*, and a genomic amplification containing sdrM, are shown for populations from intermediate passages of three independently evolved populations each of the WT and sdrM::Tn strains. c The survival fraction for the indicated isolates in multiple DLX concentrations compared to a no DLX control, measured as cfu/ml on the respective plates. Data shown are the mean ± standard deviation of three biological replicates. d Percentage of the 12 independent populations of the indicated strains that evolved DLX resistance over time in 2.5x the respective DLX MICs (0.55 µg/ml for the WT and 0.32 µg/ml for sdrM::Tn). Source data for (a, c, d) are provided in the Source Data file.

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