Fig. 3: EB1 decorates the full-length spindle MTs in activated male gametocytes.

a IFA of HA-tagged EB1 in the eb1::6HA male gametocytes during gametogenesis. Nuclei are stained with Hoechst 33342. Representative for three independent experiments. Scale bar = 5 μm. b Fluorescence microscopy observation of GFP-tagged EB1 in the eb1::gfp male gametocytes during gametogenesis. Representative for three independent experiments. Scale bar = 5 μm. c Co-staining the eb1::6HA male gametocytes with antibodies against HA and α-Tubulin during gametogenesis. Hoechst, Hoechst 33342. White arrows indicate the mitotic spindle. Representative for three independent experiments. Scale bar = 5 μm. d Ultrastructure expansion microscopy (U-ExM) of EB1 and α-Tubulin expression in the eb1::6HA gametocytes during gametogenesis. Parasites were co-stained with antibodies against HA and α-Tubulin. Representative for three independent experiments. Scale bar = 5 μm. e U-ExM of EB1 and α-Tubulin expression in the eb1::4Myc gametocytes at 4-6 mpa. The parasites were co-stained with antibodies against Myc and α-Tubulin. Representative for three independent experiments. Scale bar = 5 μm. f Model showing EB1 (red) binding the full-length MTs of eight hemispindles in activated male gametocytes. The hemispindles were indicated in red color, the axonemes in green color, and the nuclear membrane was indicated as the blue dashed line.