Fig. 4: Adipocytes are non-fibrogenic in ex vivo models.
a Immunostainings of AdipoqCre;R26mTmG explants at day 0 and day 4. Adiponectin-lineage cells in green, fibroblasts in red, and marker gene αSMA (top) and Fibronectin 1 (bottom) expression in magenta. Merged channel image of the whole explant (left), magnified area of individual channels (right). Scale bars: 100 µm in low magnification images, 20 µm in high magnification images. This experiment was repeated three times independently with similar results. b Feature plots generated from combined analysis of mcSCRBseq showing adipocyte, myofibroblast, and extracellular matrix-specific enrichment in cell type-specific clusters. c-f Adipocyte-lineage cells deposit marginal matrix proteins than fibroblasts under scarring conditions. c Schematic of in vitro matrix deposition assay and quantification using Image J. d FACS-sorted adipocytes, and fibroblasts were cultured in vitro, with and without rTGFβ1 stimulation for 72hrs, followed by decellularization and immunolabelling of deposited matrix Collagen 1 and Fibronectin 1. Scale bars: 50 µm. e,f Quantification of percent fluorescence of deposited matrix showing higher percentage of deposited matrix when compared to adipocytes, n = 3 biological replicates and 4 images of each replicate, Mean ± SD, Two-way ANOVA with 95% CI.
