Fig. 5: Effect of catecholamines (CAs) or conditioned medium from single-housed control (SHC) and chronic subordinate colony housing (CSC) mice and different adrenoceptor (AR) antagonists on in vitro chondrocyte-to-osteoblast transdifferentiation.
From: Neutrophil-derived catecholamines mediate negative stress effects on bone
a Experimental setup of testing different synthetic CAs on in vitro chondrocyte-to-osteoblast transdifferentiation. b Heatmap visualization of the effects of norepinephrine (NE), epinephrine (EPI) and dopamine (DOP) on the expression of pluripotency and osteogenic marker genes in transdifferentiating ATDC5 cells versus. respective control conditions. n = 4. c Experimental setup of testing conditioned medium of CD11b+ myeloid bone marrow cells isolated from THflox/flox/CD11b-Cre- and Cre+ SHC/CSC mice in combination with antagonists for β-Adrenoceptor (β-AR), α-Adrenoceptor (α-AR), α1-AR, α2-AR, class 1 dopaminergic receptor (D1/5) and class 2 dopaminergic receptor (D2/3/4) signaling. Expression of d, e pluripotency (d: Sex determining region Y box (Sox)2; e: Nanog) and f–h osteogenic marker genes (f: Core-binding factor alpha (Cbfa)1; g: Sp7; h: Alkaline phosphatase (Alpl)) in transdifferentiating ATDC5 cells after 24 h of osteogenic differentiation in conditioned medium with receptor antagonists. n = 6–9. Data are presented as mean + SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 versus respective SHC condition; #P ≤ 0.05, ##P ≤ 0.01 versus respective control group. n.s. not significant. Source data, exact n-numbers, exact p values and used statistical tests per panel are provided in the Source Data file.
