Fig. 6: Effects of 19 d of chronic subordinate colony housing (CSC) on bone homeostasis in Adrb2flox/flox/Col2a1-Cre (Adrb2flox/Cre) mice.
From: Neutrophil-derived catecholamines mediate negative stress effects on bone
a Experimental timeline for Adrb2flox/Cre− and Cre+ exposed to 19 d of single-housed control (SHC)/CSC conditions (partly created with BioRender.com licensed to SOR). Mice were single-housed for one week before the start of the CSC paradigm on Day 1. The aggressor mouse was changed on Days 8 and 15 and the experimental mice were tested for anxiety-like behavior in the open field/novel object (OF/NO) test on Day 19 and euthanized on Day 20. b Relative adrenal weight, c femur length, d tibia length, e trabecular thickness, f trabecular tissue mineral density (Tb. TMD), g bone volume to tissue volume ratio (BV/TV) of Adrb2flox/Cre- and Cre+ SHC/ CSC mice. n = 7–8. Data are presented as mean + SEM including individual values. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 versus respective SHC condition; #P ≤ 0.05, ###P ≤ 0.001 versus respective Adrb2flox/Cre− group. n.s. not significant. Source data, exact n-numbers, exact p values and used statistical tests per panel are provided in the Source Data file.
