Fig. 4: OR0642 in combination with dT has strong antileukemic effect in vivo.

a Schedule of the vehicle and drugs administration mice groups (vehicle (n = 8), dT 1.5 g/kg (n = 7), OR0642 40 mg/kg (n = 9) and dT 1.5 g/kg + OR0642 40 mg/kg (n = 9)) (once/day (QD) and twice/day (BID)) against CCRF-CEM leukemia bearing mice. b Effect of treatments on the quantification of whole body radiance of CCRF-CEM leukemia bearing mice (bioluminescence). The yellow arrow highlights the end of the treatment. Data are presented as the mean ± SEM and compared using two-way ANOVA test and Turkey’s multiple comparison test (vehicle (n = 8), dT (n = 7), OR0642 (n = 9) and dT + OR0642 (n = 9)). NS represents no statistical significance, *P < 0.05, and **P = 0.001. c Representative bioluminescence images of mice at day 21 (NI = non injected mouse; without graft) (n = 3). d Effect of treatments on the quantification of whole body radiance at day 21. (NI = non injected mouse; without graft). Data are presented as the mean ± SEM. Comparison of bioluminescence data at day 21 was performed using one-way ANOVA Kruskal–Wallis test and Dunn’s multiple comparison test (vehicle (n = 8), dT (n = 7), OR0642 (n = 9) and dT + OR0642 (n = 9)). NS represents no statistical significance and ***P = 0.0002. e Effect of treatments on hCD45⁺ populations determined by flux cytometry at day 21. Data are presented as the mean ± SEM. Comparison of hCD45⁺ populations was performed using one-way ANOVA Kruskal–Wallis test and Dunn’s multiple comparison test (vehicle (n = 8), dT (n = 7), OR0642 (n = 9) and dT + OR0642 (n = 9)). NS represents no statistical significance and ***P = 0.0005. f Survival analysis of CCRF-CEM leukemia bearing mice treated with vehicle and drugs. The yellow arrow highlights the end of the treatment. Median survival times were compared using Log-Rank (Mantel–Cox) Test. NS represents no statistical significance and ****P < 0.0001.