Fig. 2: Phenotypic analysis and genotype identification results for the os1900&os5100 mutant. | Nature Communications

Fig. 2: Phenotypic analysis and genotype identification results for the os1900&os5100 mutant.

From: Fertilization controls tiller numbers via transcriptional regulation of a MAX1-like gene in rice cultivation

Fig. 2

a MAX1-gRNA1 and its target sequence and mutation sites. The single MAX1-gRNA1 caused mutations in both Os1900 and Os5100. b k-mer coverage differences among the Os1900, Os5100, Os900, Os1400, and Os1500 genes in wild-type (WT, cv. Koshihikari) and os1900&os5100 mutant in rice. Vertical red lines indicate the locations of gRNA used in this experiment. y-axis: log10 (k-mer for os1900&os5100/k-mer for WT). The k value was 20. c Photographs of tiller phenotypes including WT and os1900, os1500, and os1900&os5100 mutants. Bar = 5 cm. d Tiller numbers from the 1-leaf to 10-leaf stages. The P-values for different pairs (Koshihikari vs. os1900; Koshihikari vs. os5100; Koshihikari vs. os1900&os5100) were obtained based on making generalized linear mixed models (GLMMs) and ANOVA analysis. Error bars indicate SD, n = 5 biologically independent plants. e Comparison of tiller number between WT and the os1900&os5100 mutant under four cultivation conditions, n = 4 biologically independent plants. Significance values are from Student’s t-test (two-tailed). Error bars: SD.

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