Fig. 4: Detailed gene expression of Os1900 to fertilization and regulation of tiller number under minor- and trace- fertilization conditions.
a Schematic diagram of fertilization conditions for Os1900 expression analysis in leaves. After germination, plants were cultivated without fertilizer for 7 days, and then ½ Kimura’s B solution fertilizer was applied. From the 9th day, group i was sampled daily, and groups ii-iv were fertilized again when fertilizer concentration (total dissolved solids, TDS) dropped to 90, 50, and 30 ppm, respectively. Sampling (triangles) was conducted at 0, 1, 3, 6, and 24 h after fertilization. b Analysis of Os1900 expression under different fertilizer conditions. The error bar is SD, n = 3, 4 biologically independent samples. c, d Tiller numbers of os1900, os5100 single mutant and os1900&os5100 double mutant under minor- c and trace- d fertilization condition from 4- to 9-leaf age, n = 5 biologically independent plants. Cultivation condition: see methods. The P-values for different pairs (Koshihikari vs. os1900; Koshihikari vs. os5100; Koshihikari vs. os1900&os5100) are obtained by making a GLM model and subsequent ANOVA test. Error bars: SD.
