Fig. 3: LPL is an endogenous SEL1L-HRD1 ERAD substrate in adipocytes.
From: The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes
a Immunoblot analysis following immunoprecipitation of endogenous LPL in differentiated adipocytes (3 independent repeats). b Immunoblot analysis of LPL in differentiated adipocytes pre-treated with Brefeldin A for 30 min followed by cycloheximide (CHX) treatment for the indicated times (2 independent repeats). c Immunoblot analysis of LPL in differentiated adipocytes (3 independent repeats). d–g Immunoblot analysis of LPL and other proteins in gonadal WAT (d) with quantitation shown in e-g (n = 3 mice per genotype for LPL and GLUT4 and n = 2 mice per genotype for CALNEXIN). h qPCR analysis of Lpl mRNA levels in gonadal WAT (n = 14 per WT and DKO, n = 9 for Sel1LAdipCre and n = 11 for Atg7AdipCre). Histogram was plotted as mean with SD; each data points were derived from biologically independent mice/samples. P values were derived by one-way ANOVA followed by Tukey’s test; n.s. not significant. Source data are provided as a Source Data file.
