Fig. 4: Dynamic variance explained across stem cell pseudotime.
From: A village in a dish model system for population-scale hiPSC studies
a Pseudotime appeared to define cell pluripotency as evidenced by pluripotency markers and ectodermal markers expressed higher pseudotime corresponding to spontaneously differentiated cells. b The pseudotime projected onto the UMAP of all cells. c–e Markers representative of pseudotime progression: pluripotency (POU5F1; c), Neural Ectoderm (LIX1, d) and Epidermal Ectoderm (PTN; e). f The dynamic interaction of pseudotime with the CHCHD2 gene—hiPSC line effects are larger at smaller pseudotime values (pluripotent cells) and smaller at larger pseudotime values (spontaneously differentiated cells). The band around the lines represents the standard error. hiPSC: human induced pluripotent stem cell.
