Fig. 6: The amount of TCR+TIS release is correlated with the enhanced T cell proliferation under physiological setting. | Nature Communications

Fig. 6: The amount of TCR+TIS release is correlated with the enhanced T cell proliferation under physiological setting.

From: Trogocytic molting of T cell microvilli upregulates T cell receptor surface expression and promotes clonal expansion

Fig. 6

a CTV-labeled/ anti-TCRβ-Alexa 488-stained OTII CD4+ T cells were plated on pOVA323–339/I-Ab (2 μg/ml) plus various adhesion matrices including ICAM-1, VCAM-1, and fibronectin (FN) (each 10 μg/ml) for 3 h. The release of TCRβ+ particles (TISs) was determined by confocal microscopy (a, left) and flow cytometry (a, middle). Surface TCRβ exression was also analyzed (a, right). b The cells from (a) were cultured for 6 d with fresh media and the cell division was measured. Source data are provided as a Source Data file. c The number of TCRβ+ particles at each condition (a) was quantified by CytoFLEX. Statistics was performed using one-way ANOVA post hoc Tukey’s multiple comparisons test. Results are representative of three independent experiments ± SEM. p value was indicated vs. NT.

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