Fig. 3: PtenHNP/HNP males have a less advanced PIN phenotype than Pten+/– males despite similarly reduced PTEN levels. | Nature Communications

Fig. 3: PtenHNP/HNP males have a less advanced PIN phenotype than Pten+/– males despite similarly reduced PTEN levels.

From: Hyperphosphorylated PTEN exerts oncogenic properties

Fig. 3: PtenHNP/HNP males have a less advanced PIN phenotype than Pten+/– males despite similarly reduced PTEN levels.The alternative text for this image may have been generated using AI.

a CRISPR-CAS9-targeting design of Pten hypomorphic mice. PtenHN, hypomorph Neo; PtenHNP, hypomorph Neo & PolyA. b WB of prostate lysates harvested at 2 months, probed for PTEN and activation of AKT signaling pathway. Blot is representative of at least 3 individual samples. Ponceau S (PonS) staining served as loading control. c Quantitation of PTEN level in prostate based on WB. d Percentage of prostate tubules with indicated PIN lesions at 6 months. Images depict examples of various PIN lesions. Scale bars are 250 µm. n, number of prostates. Data in (c and d) are presented as mean values ± SEM. Statistical significance was assessed by ordinary one-way ANOVA, followed by Tukey’s multiple comparisons test in (c) and Dunnett’s multiple comparisons test compared to Pten+/– in (d). Source data are provided as a Source Data file.

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