Fig. 4: PIN lesion formation in Pten+/– males is characterized by loss of PTEN catalytic function. | Nature Communications

Fig. 4: PIN lesion formation in Pten+/– males is characterized by loss of PTEN catalytic function.

From: Hyperphosphorylated PTEN exerts oncogenic properties

Fig. 4: PIN lesion formation in Pten+/– males is characterized by loss of PTEN catalytic function.The alternative text for this image may have been generated using AI.

a Immunostaining of consecutive sections of normal and PIN lesion (*) tubules of indicated genotypes stained for H&E, PTEN and P-AKTS473. Scale bar is 100 µm. b Close-up of a small lesion of epithelial cells that have lost PTEN expression and have gained P-AKTS473 expression. c Quantitation of PIN lesions with increase in P-AKTS473 expression and simultaneous loss of PTEN. 5 mice per genotype with 12-55 PIN lesions per mouse were analyzed by IF staining. Data are presented as mean values ± SEM. Statistical significance was assessed by two-tailed unpaired t-test. d WB of lysates from prostates of indicated genotype and age in months (M), probed for PTEN, AKT, P-AKTT308 and P-AKTS473. Ponceau S (PonS) staining served as loading control. N = 2 individual prostates per genotype and timepoint, except the 9-month sample (n = 1). Source data are provided as a Source Data file.

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