Fig. 7: Hyperactive WNT signaling predisposes PtenS380D mice to PIN lesions.
a Mixtures of recombinant human PTEN and β−catenin proteins subjected to immunoprecipitation with PTEN or corresponding control (anti-RFP) antibodies and analyzed by immunoblotting using PTEN and β−catenin antibodies.The blot is representative of 3 independent experiments. b Immunoblot of 2-month-old fractionated prostate lysates of indicated genotypes subjected to immunoprecipitation with PTEN or control antibody and analyzed with the indicated antibodies. Blots represent 3 independent experiments. * Marks IgG band, only visible in the nuclear fraction because of the necessary long exposure to be able to detect the PTEN band. c Percentage of prostate tubules (n = 5) with indicated PIN lesions at 6 months. PIN IV were not present and are therefore not depicted. d Immunostaining of prostates of indicated genotypes stained for total ß-catenin. Asterisks indicate epithelial cells with nuclear staining. Scale bar represents 10 µm. The percentage of epithelial cells with nuclear staining is graphed. 5 prostates per genotype were analyzed, with 137-230 cells counted per prostate. e As in (d) but now for Pten+/– PIN lesions and for Pten+/– normal surrounding tissue. Data in (c–e) are presented as mean values ± SEM. Statistical significance in (c–e) was assessed by a two-tailed unpaired t-test. Source data are provided as a Source Data file.
