Fig. 6: The CXCR4-CXCL12 axis is involved in the control of FAAH-mediated inhibition of the invasive phenotype of BC cells.
From: Identification of fatty acid amide hydrolase as a metastasis suppressor in breast cancer
Representative histograms of flow cytometric detection of cell membrane CXCR4 in FAAH-modulated T-47D and MDA-MB-231 cell lines (a) and in tumors derived from MMTV-neu:FAAH+/+ and FAAH−/− mice (b). c Scatter plot showing the inverse correlation between FAAH and CXCR4 or CXCL12 mRNA expression in BC samples according to the METABRIC dataset15. Correlative associations were calculated by the Pearson’s r. Analysis of AMD3100 treatment (d, e) and CXCL12-induced chemotaxis (e) on the invasive capacity of FAAH-modulated T-47D (d) and MDA-MB-231 (e) cells. AMD3100 (1 μM) was added to the upper compartment after cell seeding. CXCL12 (50 nM) was added to the lower compartment of the Boyden chamber containing serum-free medium. Chemotactic effect of CXCL12 in T-47D cells could not be tested due to their null invasivity towards serum-free medium. Data are presented as mean values ± SEM of n = 5 and 7 biological replicates for T-47D and MDA-MB-231 cells, respectively, and were analyzed by 1-way ANOVA with post-Tukey’s multiple comparison test. Source data are provided as a Source Data file.
