Fig. 3: HDAC9 deficiency attenuated kidney fibrosis and inflammation.
From: HDAC9-mediated epithelial cell cycle arrest in G2/M contributes to kidney fibrosis in male mice
a H&E staining, Masson´s trichrome staining, Sirius Red staining, collagen I staining, collagen IV staining and F4/80 staining were performed to assess the kidney injury, fibrosis and inflammation in different groups. Scale bar: black = 50 μm. (n = 6 mice per group). b Representative Western blot gel documents and summarized data showing the relative protein levels of Vimentin and α-SMA in the cortex of kidney from different groups of mice. (n = 6 mice per group). c–e Relative mRNA level of IL-1β, IL-6 and TNFα in the cortex of kidney from AAN mice. (n = 6 mice per group). f Representative Western blot gel documents and summarized data showing the relative protein levels of Vimentin and Collagen I in HK-2 with AA treatments. (n = 6 biologically independent experiments). g, h Relative mRNA level of IL-6 and TNFα in HK-2 with AA treatments. (n = 6 biologically independent experiments). Data are expressed as mean ± SEM (a–h). Two-way ANOVA followed by Tukey’s post-test (a–h). Source data are provided as a Source Data file.
