Fig. 1: TRABID is required for proper mitotic cell division. | Nature Communications

Fig. 1: TRABID is required for proper mitotic cell division.

From: TRABID inhibition activates cGAS/STING-mediated anti-tumor immunity through mitosis and autophagy dysregulation

Fig. 1

a Control (CTL) and Trabid KO (KO) MEFs stably expressing H2B-mCherry (to mark the chromosomes) were examined for mitotic cell division by time-lapse microscopy. Images were taken at 2 min intervals between acquisitions. Time (min) is given relative to the first time frame in late G2 phase. Mitotic errors such as chromosome bridge (arrowhead) and chromosome mis-segregation (arrow) were observed. Micronuclei in the interphase cells are marked by asterisks. Bars, 20 μm. b The duration from metaphase to mitotic exit (defined by cell re-attachment) of indicated cell types was counted. Data are mean ± SD (n = 3 independent experiments and >50 cells per group per experiment were counted). Each color represents an individual experiment. P values are determined by two-sided Student’s t-test. c–g The percentages of mitotic cells showing lagging chromosomes (c), mis-segregated chromosomes (d), chromosome bridges (e), mitotic errors (f), and cytokinesis failure (g) were counted. Data are mean ± SD (n = 3 independent experiments and >50 cells per group per experiment were counted). P values are determined by two-sided Student’s t-test. h Mitotic errors observed from HeLa cells stably expressing control or TRABID shRNA together with H2B-mCherry. TRABID expression levels and representative time-lapse images are shown in Supplementary Fig. 1i, j, respectively. Data are mean ± SD (n = 3 independent experiments and >50 cells per group per experiment were counted). P values are determined by one-way ANOVA with Tukey’s post hoc test. i, j Mitotic errors observed from HeLa cells stably expressing H2B-mCherry and transfected with TRABID siRNA (i) or TRABID siRNA together with TRABID wild type or ΔN/CS construct (j). Data are mean ± SD (n = 3 independent experiments and >50 cells per group per experiment were counted). P values are determined by two-sided Student’s t-test. The expression levels of TRABID and mutant are shown. Blots in (i), (j) are representatives of three independent experiments. Source data are provided as a Source Data file.

Back to article page