Fig. 6: TRABID knockdown in tumor cells induces an anti-tumor immune microenvironment. | Nature Communications

Fig. 6: TRABID knockdown in tumor cells induces an anti-tumor immune microenvironment.

From: TRABID inhibition activates cGAS/STING-mediated anti-tumor immunity through mitosis and autophagy dysregulation

Fig. 6

a, b Tumor volume at indicated days and tumor weight at day 15 after C57BL/6 mice inoculated with B16F10 cells expressing control or Trabid shRNAs. Data are mean ± SD (n = 6 per group). P values are determined by two-way (a) or one-way (b) ANOVA with Tukey’s post hoc test. c Kaplan–Meier survival curves for C57BL/6 mice inoculated with B16F10 cells expressing control or Trabid shRNAs. P values are determined by log rank Mantel Cox test, n = 6. d, e Tumor volume at indicated days and tumor weight at day 18 after C57BL/6 mice inoculated with B16F10 cells expressing Trabid shRNAs and reconstituted with Trabid wild type or ΔN/CS mutant. Data are mean ± SD (n = 5 per group). P values are determined by two-side Student’s t-test. f, g Tumor volume at indicated days and tumor weight at day 15 after Nude mice inoculated with B16F10 cells expressing control or Trabid shRNAs. Data are mean ± SD (n = 5 per group). P values are determined by two-way (f) or one-way (g) ANOVA with Tukey’s post hoc test. h–o Flow cytometry analysis for the percentages of indicated types of tumor-infiltrating immune cells in the tumor tissues taken from day 15 of C57BL/6 mice after inoculation with B16F10 cells expressing control or Trabid shRNAs. Data are mean ± SD (n = 4 per group). P values are determined by one-way ANOVA with Tukey’s post hoc test. (p, q) Tumor volume at indicated days and tumor weight at day 15 after C57BL/6 mice inoculated with B16F10 cells expressing Trabid shRNA and treated with vehicle or C176. Data are mean ± SD (n = 5 per group). P values are determined by two-sided Student’s t test. Source data are provided as a Source Data file.

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