Fig. 6: dParp writes serine-ADP-ribosylation in a dHpf1-dependent manner. | Nature Communications

Fig. 6: dParp writes serine-ADP-ribosylation in a dHpf1-dependent manner.

From: Serine ADP-ribosylation in Drosophila provides insights into the evolution of reversible ADP-ribosylation signalling

Fig. 6: dParp writes serine-ADP-ribosylation in a dHpf1-dependent manner.The alternative text for this image may have been generated using AI.

A Mono-Ser-ADPr of H3 histone peptide (aa 1–21) was obtained by incubation with recombinant hPARP1:hHPF1 complex, or recombinant dPARP1:dHPF1 complex using 32P-NAD+ as the ADP-ribose donor. PARP reactions were stopped by the addition of olaparib. The experiment was repeated independently three times with similar results. B ADP-ribosylated histone H3 peptides from the (A) were purified from the reaction and treated with recombinant hARH3 to confirm the presence of Ser-ADPr. The experiment was repeated independently three times with similar results.

Back to article page