Fig. 6: Quantitative proteomics analysis for exploring the antitumor mechanism of MiBaMc combined with aPDL1.

a Hierarchical clustering analysis of 92 differentially expressed proteins in tumor tissues after treatment with PBS and MiBaMc + PTDT + aPDL1 (n = 3 mice). The heatmap shows significantly upregulated (70) and downregulated (22) proteins (fold change ≥ 1.5 and P-value < 0.05). Fold change values were color-coded from purple (downregulation) to red (upregulation). b GO terms enrichment analysis of differentially expressed proteins. The abscissa shows enriched GO function classifications, namely three major categories: biological functions, cellular components, and molecular functions. c Venn diagram of the non-overlapped biological effect associated with hypothermia-ROS synergistically induced cell death and overlapped biological effect associated with bacteria membrane induced immune response. d Histograms of differential proteins related to heat shock, oxidative stress, cell death, inflammatory response, bacteria infection, and immune response according to the GO annotation (n = 3 mice). e Differentially expressed protein interaction networks. The circle represents the gene and the line represents the protein-protein interaction. Data are presented as mean values ± SD. Source data are provided as a Source Data file.