Fig. 3: AI-PrL pathway affected the activity of stable neurons in PrL during the SN-trials.

A Stable and dynamic neurons defined by longitudinal registration of calcium imaging over two consecutive days (SN-trials with and without light stimulation) from animals expressing only control viruses, not opsin. Even without optogenetic manipulation, several PrL neurons showed the same neuronal activity patterns during SN-trials on two consecutive days (defined as “stable neurons”), while others showed different activity patterns (defined as “dynamic neurons”). B Significant differences in the patterns of PrL activity changes between SN-trials on two consecutive days were observed between stressed and unstressed dams expressing control viruses (Chi-squared test). C Scheme of our hypothesis regarding the effect of the AI-PrL pathway on PrL activity during SN-trials. D Significant differences in the patterns of PrL activity changes from SN-trials without light stimulation to SN-trials with light stimulation were observed between unstressed dams expressing control viruses and eNPHR (Chi-squared test). E Significant differences in the patterns of PrL activity changes from SN-trials without light stimulation to SN-trials with light stimulation were observed between stressed dams expressing control viruses and Chrimson (Chi-squared test). **p < 0.01. See Supplemental Table 3 for details on the statistical analyses.