Fig. 5: ABCs express higher levels of Fc γ receptor IIB and bind higher proportions of immune complexes than other B cells subsets. | Nature Communications

Fig. 5: ABCs express higher levels of Fc γ receptor IIB and bind higher proportions of immune complexes than other B cells subsets.

From: Age-associated B cells predict impaired humoral immunity after COVID-19 vaccination in patients receiving immune checkpoint blockade

Fig. 5

a Violin plots displaying the expression levels of FCGR2B on different B cell subsets. b Representative flow cytometry contour plots displaying immune complex binding (contour plots in green) with or without a previous incubation with Fc block by age-associated B cells (ABCs), memory B cells (MBCs), naïve B cells and plasmablasts (PBs). c Representative flow cytometry contour plots displaying cytokines production (IL-6 and TNF, PMA/ionomycin stimulation for 5 h, 37 °C, brefeldin A) by ABCs, MBCs, naïve B cells and PBs. d Frequencies of ICs binding cells in different B cell subsets. e Frequencies of cells with IL-6- and TNF-secreting capacity in different B cell subsets. Where specified, statistical significance between groups was determined using one-way ANOVA. d, e HC = 6, IEI = 6, ICB = 6 biologically independent samples.

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