Fig. 5: Abrogation of NF-κB signaling aggravates cholestasis in Mdr2−/− mice.
From: Colitis ameliorates cholestatic liver disease via suppression of bile acid synthesis
a Western blot of liver phosphorylated NF-κB P65 (WT, n = 3; Mdr2−/−, n = 5; WT + DSS, n = 3; Mdr2−/−+DSS, n = 5). b Spearman correlation heatmap of RNA-seq analysis demonstrates negative correlation between liver Nfkb and BA metabolism genes(Mdr2−/−, n = 10; Mdr2−/−+DSS, n = 10). c Representative images of Mdr2−/− and Mdr2−/−NemoΔhepa mice. d Body weight of Mdr2−/− and Mdr2−/−NemoΔhepa mice (Mdr2−/−, n = 7; Mdr2−/−NemoΔhepa, n = 5); unpaired two-tailed Student’s t-test (Mdr2−/− vs Mdr2−/−NemoΔhepa, P < 0.0001). e Serum ALT, AP and AST levels (Mdr2−/−, n = 7; Mdr2−/−NemoΔhepa, n = 5); unpaired two-tailed Student’s t-test (ALT: Mdr2−/− vs Mdr2−/−NemoΔhepa, P = 0.0008; AST: Mdr2−/− vs Mdr2−/−NemoΔhepa, P < 0.0001); Two-tailed Mann–Whitney test (AP: Mdr2−/− vs Mdr2−/−NemoΔhepa, P = 0.0025). f The percentage of hepatic neutrophils to CD45+ leukocytes (Mdr2−/−, n = 4; Mdr2−/−NemoΔhepa, n = 5); unpaired two-tailed Student’s t-test (Mdr2−/− vs Mdr2−/−NemoΔhepa, P = 0.0064). g Representative images of liver H&E, CK19 and Sirius red stainings (Mdr2−/−, n = 8; Mdr2−/−NemoΔhepa, n = 6) (scale bar, 100 µm). h Liver mRNA expression of fibrotic markers (Mdr2−/−, n = 5; Mdr2−/−NemoΔhepa, n = 7); unpaired two-tailed Student’s t-test (Mdr2−/− vs Mdr2−/−NemoΔhepa: Col1a1, P = 0.0013; Col1a2, P = 0.0006; Col3a1, P = 0.035; Mmp2, P = 0.0047; Timp1, P = 0.0181; αSma, P = 0.0025; Cd34, P = 0.0247; Thy1, P = 0.0164; Elastin, P = 0.0002); Two-tailed Mann–Whitney test (Mdr2−/− vs Mdr2−/−NemoΔhepa: Desmin, P = 0.0303; Msln, P = 0.0051). i Western blot of liver Cyp7A1 and αSMA (Mdr2−/−, n = 5; Mdr2−/−NemoΔhepa, n = 8); unpaired two-tailed Student’s t-test (αSMA: Mdr2−/− vs Mdr2−/−NemoΔhepa, P = 0.0003); Two-tailed Mann–Whitney test (Cyp7A1: Mdr2−/− vs Mdr2−/−NemoΔhepa, P = 0.0016). j Bile acids measurement of liver (Mdr2−/−, n = 5; Mdr2−/−NemoΔhepa, n = 3) and serum (Mdr2−/−, n = 5; Mdr2−/−NemoΔhepa, n = 4); unpaired two-tailed Student’s t-test (Liver total BAs: Mdr2−/− vs Mdr2−/−NemoΔhepa, P = 0.0359); Two-tailed Mann–Whitney test (Serum total UBAs: Mdr2−/− vs Mdr2−/−NemoΔhepa, P = 0.0095). All data are mean ± SEM and considered significant at *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Source data are provided as a Source Data file.
