Fig. 6: VD-deficient cord blood monocytes induce adipocyte IR.
From: Embryonic vitamin D deficiency programs hematopoietic stem cells to induce type 2 diabetes
A Cord blood serum 25(OH)D levels from 30 healthy pregnant women at delivery. Mean and 95% confidence interval. B Correlation between cord blood serum 25(OH)D levels and birth weight using Spearman’s correlation coefficient. C Correlation between cord blood serum 25(OH)D levels and change in insulin-stimulated 2-DG uptake in 3T3-L1 adipocytes cultured in conditioned media of cord blood monocytes using Spearman’s correlation coefficient. Western blot analysis of insulin signaling pathway from 3T3-L1 adipocytes exposed to cord blood monocytes (D) and from Jarid2/Mef2/PGC1α network-related proteins of cord blood monocytes (E) (n = 4/group from two independent experiments). F Quantitative RT-PCR of mRNA expression of Jarid2/Mef2/PGC1α network-related genes from cord blood monocytes stratified by 25(OH)D level (25(OH)D < 20 ng/mL and ≥20 ng/mL) (n = 4/group). G Correlation between cord blood serum 25(OH)D level and serum miR-106b-5p expression (n = 30) using Spearman’s correlation coefficient. H Insulin-stimulated 2-DG uptake in 3T3-L1 adipocytes transfected with miR-106b-5p antagomir and cultured in conditioned media from blood monocytes with 25(OH)D < 20 ng/mL or ≥20 ng/mL (n = 6/group). I Mechanistic schematic diagram Created with BioRender.com. Data presented as mean ± SEM. F *P < 0.05; **P < 0.01; ***P < 0.001 by two-tailed unpaired t test. H **P < 0.01; ***P < 0.001 by one-way ANOVA followed by Tukey’s multiple comparison test. Actual P values are shown in the source data file.
