Fig. 1: Map-based cloning of the major gene in qCS11-jap. | Nature Communications

Fig. 1: Map-based cloning of the major gene in qCS11-jap.

From: The COG1-OsSERL2 complex senses cold to trigger signaling network for chilling tolerance in japonica rice

Fig. 1: Map-based cloning of the major gene in qCS11-jap.The alternative text for this image may have been generated using AI.

a Chilling tolerance of 93-11 (indica) and of chromosome segment substitution line 28 (CSSL-28). The survival rates were determined after a 4-week recovery period. Data are means ± SD (two-tailed Student’s t-test; n = 3 biological replicates). Scale bars = 6 cm. b Location of qCS11-jap on chromosome 11 revealing by LOD score plots. c Fine mapping of the qCS11-jap locus. Left, high resolution mapping. STS11-5, STS11-11, MK21.21, MK21.32, MK21.9, MK22.3, STS11-12 and STS11-13 were molecular markers (Supplementary Data 5). Right, survival rates of six recombinants (R1–R6) and the parents. Data are means ± SD (n = 3 biological replicates). d Chilling tolerance of 93-11 and a homozygous recombinant line (R2-7-4). Data are means ± SD (two-tailed Student’s t-test; n = 3 biological replicates). Scale bars = 6 cm. e The expression levels of ORF9 (LOC_Os11g36200) in 93-11 and Nipponbare (NIP) with different treatment times at 4 °C. Data are means ± SD (n = 3 biological replicates). See also Supplementary Fig. 1. Source data are provided as a Source Data file.

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