Fig. 2: COG1 is essential for chilling tolerance in japonica rice.

a Identification of cog1-1 and cog1-2 via sequencing. PAM, protospacer adjacent motif. b The expression levels of COG1 in ZH11 and two COG1-overexpression lines (OE1 and OE4). Data are means ± SD (n = 3 biological replicates). c, d The chilling tolerance of COG1 mutants (cog1-1 and cog1-2) (c) and COG1-overexpression lines (d) in the ZH11 background. The seedlings of mutants and overexpressed lines were treated at 4 °C for 72 h and 80 h, respectively. The survival rates were determined after a 4-week recovery period. Data are shown as the means of three replicates ± SD (two-tailed Student’s t-test). Bar = 6 cm. e The chilling tolerance of COG1::COG1-HA transgenic lines (Line2 and Line4) in the indica 93-11 background. The seedlings were treated at 4 °C for 44 h. Bar = 6 cm. Data are means ± SD (two-tailed Student’s t-test; n = 3 biological replicates). f The chilling tolerance of COG1-overexpression lines (OX1 and OX8) in the 93-11 background. The seedlings were treated at 4 °C for 44 h. Bar = 6 cm. Data are means ± SD (two-tailed Student’s t-test; n = 3 biological replicates). See also Supplementary Fig. 2. Source data are provided as a Source Data file.