Fig. 4: COG1 encodes a plasma membrane and ER localized LRR-RLP. | Nature Communications

Fig. 4: COG1 encodes a plasma membrane and ER localized LRR-RLP.

From: The COG1-OsSERL2 complex senses cold to trigger signaling network for chilling tolerance in japonica rice

Fig. 4

a Schematic of COG1 protein structure. Amino acid residues 1 to 53 comprise the signal peptide (SP), and residues 118 to 534 form 17 LRR domains. See also Supplementary Fig. 3a. b Localization of COG1 in rice protoplasts, plasma membrane (upper panel) and ER (down panel). PIP2-mCherry is a marker for the plasma membrane and ER. Scale bar = 10 μm. c Localization of COG1 revealing by immunoblotting assay. The COG1-HA fusion protein in COG1::COG1-HA transgenic rice (COG1-HA-L1) in the ZH11 background was detected by anti-HA antibody. H+-ATPase, BiP, actin, and histone 3 (H3) were used as markers of the plasma membrane protein (PM), ER protein, cytoplasmic protein (Cyt), and nuclear protein (Nuc), respectively. d COG1 accumulation in COG1-HA-L1 exposed to chilling treatment for 0, 12, 24, and 48 h. The ratio of the intensity of the COG1-HA band to that of the rubisco band at 0 h was set as 1.0. Similar results of b–d were observed from three independent biological repeats. Source data are provided as a Source Data file.

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